A new route will open on the London Overground from Sunday (31 May), which will see trains travel between Liverpool Street and Enfield Town.
The line will pass through Enfield stations including Bush Hill Park, Edmonton Green and Silver Street.
Another route to come into effect on 31 May will travel through Turkey Street and Southbury Stations on the Chestnut line, with trains provided by Abellio Greater Anglia and customers charged Transport for London (TfL) pay-as-you-go fares.
TfL said 80% of pay-as-you-go fares are set to fall.
London Rail managing director, Mike Brown, said: “We are very pleased to take over responsibility for these routes into and out of Liverpool Street and, over time, look forward to improving the services for the passengers using them.
“Just as on London Underground, people will see staff at our stations at all times when trains are running - providing reassurance to the public as well as a turn up and go service for disabled customers.
“Passengers will also benefit from new fares which, in the most cases, will save them money, with Oyster and contactless payment continuing to provide the best value.”